Laura Curti

Post Doc

Research Lines

Genomic Science

Center

CGS@SEMM Milano

Contacts

laura.curti@iit.it

About

Education

2016  Ph.D in Pharmaceutical Sciences at University of Geneva, Switzerland.

2010  Master Degree in Applied Biology to Biomedical Research at Insubria University, Varese, Italy.

2007  Bachelor Degree in Medical Biology at Insubria University, Varese, Itay.

 

Professional Experience

November 2016 present           Post-doc, Cancer Biology group, Center for Genomic Science at IIT@SEMM, Milan, Italy.

January 2012 April 2016           PhD student, Doctoral program in Pharmaceutical Sciences,

                                                      Prostate Cancer Biology group at Institute of Oncology Research (IOR), Bellinzona, Switzerland.

April 2010 – December 2011       BiologistProstate Cancer Biology group at Institute of Oncology Research (IOR), Bellinzona, Switzerland.

Februry 2009 – April 2010           Visiting StudentProstate Cancer Biology group at Institute of Oncology Research (IOR), Bellinzona, Switzerland.

March 2007 – September 2007   Visiting Student, Laboratory of General Pathology at Insubria University of Varese, Busto Arsizio, Italy.    

Projects

The Myc protein is a multifunctional transcription factor able to regulate several cellular processes. Frequently upregulated in cancerous lesions, Myc overexpression not only causes aberrant cell growth and proliferation, but also triggers a strong DNA Damage Response (DDR), partially due to replication stress and the consequent accumulation of DNA damage at the level of stalled replication forks. Although the therapeutic targeting of Myc in cancer is highly studied, Myc-targeted drug efficacy and development still have many structural and functional challenges to be overcome. For these reasons, the identification of novel regulators of Myc activities can be exploited for therapeutic purposes.

In order to dissect the Myc-induced DDR and to uncover potential druggable regulators of this response, we set up a high-throughput RNA Interference screen based on the detection of the DNA damage marker gH2A.X at the single cell level. In order to control Myc activity, we took advantage of a conditional cell line in which Myc is fused to the Estrogen Receptor (ER) and can be quickly activated upon 4-hydroxy-tamoxifen (OHT) treatment. Through this screen, we identified both positive and negative regulators of Myc-induced DDR, and several of the candidates that, once inactivated synergized with Myc in inducing DNA damage, showed also synthetic lethality. Among these candidates that may protect Myc-overexpressing cells from broad replicative stress, we focused our attention on factors with a critical role in maintaining genomic stability. In particular, through loss of function experiments, genome wide transcriptional analysis, replication forks monitoring, and the usage of DSBs reporter systems at single cell level, we are interested in defining how these factors help Myc-overexpressing cells to overcome replication stress and genomic instability.

Defining the interplay between Myc and these novel factors will help us to unveil new potential therapeutic targets, whose inhibition could be the basis of specific therapeutic approaches for Myc-driven cancers.

IIT Publications

  • 2017
  • Croci O.iit, De Fazio S.iit, Biagioni F.iit, Donato E.iit, Caganova M.iit, Curti L.iit, Doni M., Sberna S.iit, Aldeghi D.iit, Biancotto C.iit, Verrecchia A., Olivero D., Amati B.iit, Campaner S.iit
    DOI

    Transcriptional integration of mitogenic and mechanical signals by Myc and YAP

    Genes and Development, vol. 31, (no. 20), pp. 2017-2022
  • 2016
  • Albino D., Civenni G., Dallavalle C., Roos M., Jahns H., Curti L., Rossi S., Pinton S., D'Ambrosio G., Sessa F., Hall J., Catapano C.V., Carbone G.M.
    DOI

    Activation of the Lin28/let-7 axis by loss of ESE3/EHF promotes a tumorigenic and stem-like phenotype in prostate cancer

    Cancer Research, vol. 76, (no. 12), pp. 3629-3643
  • 2012
  • Albino D., Longoni N., Curti L., Mello-Grand M., Pinton S., Civenni G., Thalmann G., D'Ambrosio G., Sarti M., Sessa F., Chiorino G., Catapano C.V., Carbone G.M.
    DOI

    ESE3/EHF controls epithelial cell differentiation and its loss leads to prostate tumors with mesenchymal and stem-like features

    Cancer Research, vol. 72, (no. 11), pp. 2889-2900
  • Babbio F., Pistore C., Curti L., Castiglioni I., Kunderfranco P., Brino L., Oudet P., Seiler R., Thalman G.N., Roggero E., Sarti M., Pinton S., Mello-Grand M., Chiorino G., Catapano C.V., Carbone G.M., Bonapace I.M.
    DOI

    The SRA protein UHRF1 promotes epigenetic crosstalks and is involved in prostate cancer progression

    Oncogene, vol. 31, (no. 46), pp. 4878-4887