Nunzio Del Gaudio

About

Education

31/10/2016 PhD in Biochemistry and Biotechnology, University of Campania “L. Vanvitelli” (Italy)

26/03/2013 Master's degree in Medical Biotechnology, University of Naples “Federico II” (Italy)

17/12/2010 Bachelor degree in Biotechnology for Health, University of Naples “Federico II” (Italy)

Research experiences

04/03/2017 – present Postdoc at Italian Institute of Technology- Center for Genomic Science

Research activity: Epitranscriptome regulation of RNA dynamics and their alteration in cancer 

15/05/2017-28/02/2018 Postdoc at University of Campania “L.Vanvitelli”, Napoli (Italy)

Research activity: Identification and characterization of novel epigenetic targets with a role in the establishment and/or maintenance of human leukemia

01/11/2013-31/10/2016 PhD student at University of Campania “L.Vanvitelli”, Napoli (Italy)

Research activity: Identification and validation of novel epigenetic targets involved in the human acute myeloid leukemia

01/04/2016–31/07/2016 Visiting PhD student at RadBoud University, Nijmegen (Netherlands)

Research activity: Identification of new chromatin target interactors by mass spectrometry-based interaction proteomics

01/03/2012–31/08/2012 Master's degree internship at Univeristy of Amsterdam, Amsterdam (Netherlands)

Research activity: Role of ANF enhancers in regulating ANF expression in embryonic mouse heart

Technical skills and competences

Molecular cloning. Preparation, extraction, purification and analysis of DNA and/or mRNA from eukaryotic cells. PCR technology: amplification of DNA by PCR, PCR mutagenesis, RT-PCR, real-time PCR. Cell culture of primary and transformed mammalian cells. Stable and transient cell transfection, retrovirus and lentivirus production and transduction. Proliferation assay (soft agar, clonogenic assay), apoptosis assays (annexin V and tunel assays), caspase activity assay, cell viability assay (MTT), reporter assays (luciferase and galactosidase). In vitro transcription and translation assay. Protein expression analysis using common electrophoresis techniques, Western blot. Protein interaction technologies: GST, GFP, MBP pulldown, co-immunoprecipitation assay. Post-translational modification assays such as λ-phosphatase assay (λ-PPase), ubiquitination assay, SUMOylation assay. Cell cycle profiles, apoptosis and differentiation by FACS analysis. Analysis of protein-DNA interaction by chromatin immunoprecipitation, analysis of protein-RNA interaction by RNA immunoprecipitation. Microarray and RNA-Seq. ChIP-seq .Immunofluorescence assay. Mass spectrometry-based interaction proteomics.

Awards

17-12-2015 Recipient of a “Blueprint Epigenome Exchange Program” travel grant

Conferences

COST: Chromatin, Epigenome and Drug Discovery Training School (Napoli IT, 2016)

The 4th BLUEPRINT Consortium Meeting (Cambridge UK, 2015)

4th Oxford Symposium - New Frontiers in Epigenetics (Oxford UK, 2015)

HDAC inhibitors - Lessons learned and challenges for the future (Napoli IT, 2015)

The 3rd BLUEPRINT Consortium Meeting (Roma IT, 2014)

Projects

Exploring the functional role of the m6A in regulating MYC-influenced RNA dynamics:

• exploring the impact of (context specific) m6A mark and its molecular determinants on shaping RNA dynamics
• investigating the involvement of m6A and its molecular determinants in the MYC-driven transcriptional response, possibly leading to the identification of specific genes with therapeutic potential in MYC-driven tumor.